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The Angiogenic Effect Of Micropuncture On Arterial And Venous Endothelial Cells
MohammadHossein Asgardoon, MD, MPH, Jessica El-Mallah, DO, Jazzmyn Dawes, bs, Mary Landmesser, bs, Dino Ravnic, DO, MPH.
penn state university, hershey, PA, USA.
Purpose:Scaffolds are essential in reconstructive surgery and tissue engineering, yet their success is often limited by delayed vascularization post-implantation. Micropuncture (MP), a novel technique using a 60-µm needle to precisely perforate vessel walls, aims to induce angiogenesis and expedite scaffold vascularization. Early in vivo studies suggest venous MP may promote faster capillary growth than arterial MP, potentially through increased release of pro-angiogenic factors from venous endothelial cells (ECs). Methods:Arterial and venous ECs were extracted from rat aorta and vena cava, respectively, and seeded onto cell culture inserts. After confluence, 20 MPs were applied per insert; controls received no MPs. Tube formation was quantified at 24 hours using Artificial Intelligence, supernatant analyzed via a 20-protein antibody array, and mRNA sequencing performed. Data were processed using ImageJ, GraphPad Prism, and R. A p-value < 0.05 was considered significant. Results:Venous MP resulted in more tube formation and increased total tube length compared to controls, with no significant differences seen in arterial cells (Fig1A). Venous MP was associated with a more pro-angiogenic profile, with 18/20 proteins increased versus 13/20 in arterial MP (Fig1B). Notable upregulated genes included Ccl2, Timp1, Il6, and Lep, while Cxcr2, Pgf, Egf, Cxcl1, and Ccl5 were downregulated(Fig1C). Conclusion:MP triggers differential angiogenesis, with venous ECs releasing more pro-angiogenic factors post-MP, leading to enhanced capillary formation. Future studies will identify key proteins for promoting scaffold vascularization.
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