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How Flow Dynamics Influence Angiogenic Factor Release In Endothelial Cells Undergoing Micropuncture
MohammadHossein Asgardoon, MD, MPH, Shailaja Agrawal, PhD, Mary Landmesser, bs, Dino Ravnic, DO, MPH.
penn state university, hershey, PA, USA.
PURPOSE: Poor vascularization limits graft survival in reconstructive surgery. Micropuncture (MP) is a novel approach designed to enhance angiogenesis by using a 60-µm diameter needle to precisely perforate blood vessel walls and stimulate microvascular growth. Static in vitro studies suggest venous MP expedites capillary formation more than arterial MP through angiogenic factor release, though the role of flow in this process remains unclear. We hypothesize that flow further enhances secretion of angiogenic factors after MP in both arterial and venous endothelial cells (ECs).
METHODS: Arterial and venous ECs were extracted from rat aorta and vena cava, seeded onto culture inserts, and subjected to 40 MPs. Non-MP cells served as controls. Cells were then transferred to a microslide channel under continuous flow (1.5 ml/min). Supernatants were analyzed after 24 hours with a semi-quantitative antibody array. Fold changes in factor secretion were calculated relative to non-MP controls.
RESULTS: Under static conditions, venous MP showed >1-fold change in 85% of angiogenic factors, compared to 55% for arterial MP (Table1). Flow further amplified venous factor release following MP, with 40% of factors showing >1.5-fold change under flow, compared to 25% in static conditions (Table1).
CONCLUSION:MP stimulates pro-angiogenic factor release, particularly from venous ECs. Flow conditions amplify this effect, suggesting a promising approach for enhancing vascularization in clinical applications.
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