Back to 2025 Resident Posters
Comparative Analysis Of Acellular Dermal Matrices As A Vehicle For Stem Cell Therapy Delivery
Margaret W. Lieb, MD, MS, Michelle Randle, MS, Carrie E. Harrison, BS, Michael W. Neumeister, MD, FRSCS, FACS.
Souther Illinois University School of Medicine, Springfield, IL, USA.
PURPOSE: Chronic wounds pose a significant clinical challenge, driving the need for innovative wound healing strategies. Acellular dermal matrices (ADMs) have gained attention as potential scaffolds for stem cell therapy. This study compares four ADMs from different sources—human, bovine, porcine, and fish—in terms of their ability to support stem cell therapy for wound healing.
METHODS: ADM A, the most processed, lacked a basement membrane and derived from human, bovine, and porcine dermis. ADM B and D, moderately processed and sourced from human dermis, retained a basement membrane. ADM C, from fish dermis, was minimally processed without a basement membrane. All ADMs except ADM D required rehydration. In vitro evaluations included ToxiLight assays for cell viability, MTT assays for proliferation, and DAPI staining for cell visualization. Murine epithelial stem cells (ESCs) were seeded on each ADM, and responses were compared to control groups without stem cell seeding.
RESULTS: ToxiLight assays indicated all ADMs supported cell viability, with ADM A and C showing the highest levels. ADM B and C demonstrated the most significant cell proliferation by day 9. DAPI staining confirmed ESC attachment and migration on all ADMs.
CONCLUSION: ADM C and A showed superior performance in promoting cell viability and proliferation, suggesting that less processed ADMs may be more effective for stem cell therapy. ADMs requiring rehydration and including xenograft components also enhanced ESC viability, while the presence of a basement membrane had no significant impact. These findings warrant further in vivo studies to advance clinical applications.
Back to 2025 Resident Posters
