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Flow Cytometric Analysis Of Adipose-derived Stromal Vascular Fraction Harvested Using Two Common Liposuction Cannula From Different Donor Sites In Autologous Fat Graft Surgery
Abtin Shams, MD1, Babak Nikoumaram, MD
2, Sona Zare, PhD
3.
1University of Colorado Anschutz Medical Campus, Aurora, CO, USA,
2Islamic Azad University Tehran Medical Sciences, Tehran, Iran, Islamic Republic of,
3Tehran University of Medical Sciences, Tehran, Iran, Islamic Republic of.
Background: Lipoaspirates, enriched in stromal vascular fraction (SVF) cells, demonstrated enhanced fat graft retention in autologous fat graft surgeries. Although several studies have evaluated the impact of different harvesting methods, cannula types, collection sites; an optimal approach remains debated. This study compared the Tonnard cannula, featuring 1mm elevated sharp microports, with the Sorensen cannula which has beveled microports, to assess their impact on SVF cell yield and viability across various donor sites.
Method: Lipoaspirates were harvested using suction-assisted liposuction from 12 healthy donors undergoing autologous fat graft surgery. The Tonnard cannula was employed on the right side, including the flank, inner thigh, and inner knee, while the Sorensen cannula was utilized on the left. Lipoaspirates were enzymatically digested, and the isolated SVFs were analyzed by flowcytometry to assess total cell yield, viability (Annexin V PI staining), reactive oxygen species (DCFH-DA staining), and stem cell CD marker expression (CD105, CD73, CD90, CD45, and CD11b).
Result: The Tonnard cannula yielded significantly higher viable SVF cells and positive CD markers expression (P<.05) irrespective of the suction region. The flank area consistently provided the highest viable cell concentration with both cannulas.
Conclusion: Lipoaspirate extracted using the Tonnard cannula from the flank provided the highest viable cell count and stem cell CD marker expression. Future research will expand the CD marker, conduct gene expression analysis, and assess the functionality of the cells in vitro.
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