American Association of Plastic Surgeons
AAPS Home AAPS Home Past & Future Meetings Past & Future Meetings

Back to 2022 Posters


Peripheral Nerve Regeneration In MRL/MpJ Mice
David Milek, MD, Scott R. Echternacht, MD, Dalton LaBarge, BA, Jonnby LaGuardia, BS, Howard N. Langstein, MD, Jonathan I. Leckenby, MD, PhD.
University of Rochester, Rochester, NY, USA.

PURPOSE: Peripheral nerve injury (PNI) outcomes remain largely unchanged for decades. Scar formation following PNI poses a physical barrier to axonal regeneration. Murphy Roths Large (MRL/MpJ) mice have demonstrated minimal scar formation in wound and tendon healing. This study seeks to evaluate whether this attribute improves peripheral nerve regeneration.
METHODS: Six-week old male mice were divided into two groups: 1) Nerve Repair (NR, n=60) and 2) Nerve Graft (NG, n=60). The C57BL/6J was compared to the MRL/MpJ strain. The right sciatic nerve was either divided and microsurgically repaired (NR) or, had a 5mm segment excised, reversed and re-interposed (NG). The Sciatic Functional Index (SFI) was used to record functional recovery at post-operative weeks (POW) 1, 3, 6, 9, and 12. Sciatic nerves were harvested and processed to calculate axon counts and the 'g' ratio for each end-point.
RESULTS: The MRL/MpJ strain demonstrated significantly superior SFI scores at POW 1, 3 (NR group) and at POW 6 (NG group). Axon counts were significantly higher in the C57BL/6J strain at POW 3 and 6 (NR group), and at POW 6, 9, and 12 (NG group). The MRL/MpJ strain demonstrated a significantly better axonal myelination at POW 3, 6, 9, and 12 (DR group) and at POW 6 and 12 (NG group).
CONCLUSION: This study demonstrates superior axonal regeneration in the MRL/MpJ strain. The results support the notion that the quality is more important than the quantity of regenerating axons. Future work implementing a mechanistic that targets nerve scarring is warranted.


Back to 2022 Posters